Penetration depth of evanescent field



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) microscopy is the job of an temporary rise for the excitation of fluorophores alternatively of exploitation direct light. A place of the evanescent wave, which arises from the glass/water or glass/specimen interface, is that its propagation in z-direction gradually degrades, modification its ability degradation into the specimen to some one c nanometers. This allows fluorophores to be exclusively excited in a small product of the cell placed enveloping to the glass/water or glass/specimen interface.
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Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results: Leica Microsystems


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Optics - Theoretical penetration limit for evanescent waves - Physics Stack Exchange


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1$ to a environs with refractive index 1$ to a environs with refractive index $1$ - see schematic below. Using Fresnel equations one gets the onslaught depth $$d = \frac,$$ wherever $k_x$ and $k_y$ are the perpendicular components of the motion vector, and $\lambda$ is the wavelength (in the vacuum). At smallest in theory, it is possible to have an impermanent rise of an arbitrary depth abasement $d$.$ - see schematic below. Using Fresnel equations one gets the onslaught depth $$d = \frac,$$ wherever $k_x$ and $k_y$ are the perpendicular components of the motion vector, and $\lambda$ is the wavelength (in the vacuum). At smallest in theory, it is possible to have an impermanent rise of an arbitrary depth abasement $d$.
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